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KMID : 1377020140110050387
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Tissue Engineering and Regenerative Medicine
2014 Volume.11 No. 5 p.387 ~ p.396
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Three dimensional reconstruction of bone-cartilage transitional structures based on semi-automatic registration and automatic segmentation of serial sections
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Hua Guo
Zheng-Wei Xu
Bao-Rong He
Ding-Jun Hao
Wei-Guo Bian
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Abstract
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A well-defined three-dimensional (3-D) reconstruction of bone-cartilage transitional structures is crucial for the osteochondral restoration. This paper presents an accurate, computationally efficient and semi-automated algorithm for the alignment and segmentation of two-dimensional (2-D) serial to construct the 3-D model of bonecartilage transitional structures. Entire system includes the following five components: (1) image harvest, (2) image registration, (3) image segmentation, (4) 3-D reconstruction and visualization, and (5) evaluation. A computer program was developed in the environment of Matlab for the semi-automatic alignment and automatic segmentation of serial sections. Semi-automatic alignment algorithm based on the position’s cross-correlation of the anatomical characteristic feature points of two sequential sections. A method combining an automatic segmentation and an image threshold processing was applied to capture the regions and structures of interest. SEM micrograph and 3-D model reconstructed directly in digital microscope were used to evaluate the reliability and accuracy of this strategy. The morphology of 3-D model constructed by serial sections is consistent with the results of SEM micrograph and 3-D model of digital microscope.
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KEYWORD
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3-D reconstruction, alignment, segmentation, morphology, serial sections
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